Eun Jung Lee
|Office:||615 Fenster Hall|
Dr. Lee has spearheaded in the development of in vitro tissue models and novel bioreactors in the field of cardiovascular tissue engineering in the past several years. She has developed spontaneously beating heart chambers exhibiting key characteristics of native heart for the first time, which is truly novel and powerful for answering questions that cannot easily be approached in vivo. She has also developed a uniaxial and a biaxial stretching device, which can be used to study the impact of mechanical stimulation on engineered cardiac tissues. More importantly, she has developed a novel flow bioreactor, which allows culture of microvasculature in vitro under the influence of flow, which is critical for any functional tissues. To the best of her knowledge, this was the first gel-based flow bioreactor, which provide a new basis for subsequent co-culture studies with various cell types to develop complex engineered tissue constructs with vascularization capacity, which is extremely critical for not only cardiac tissues, but also other tissues to be truly functional replacement tissues. With her expertise with vascular cells and microvasculature, she created tools using biochemical and shear stress conditions to guide and support the growth and development of pulmonary epithelial cells, as well as endothelium cells in the decellularized engineered lung, making it possible for lung regeneration in the laboratory. This study led her to become a co-author on a Science paper. Dr. Lee has expanded her interest to using stem cells for cardiac regeneration and for diabetes treatment. She recently discovered and derived a pure population of islet endothelial cells from mouse embryonic stem cells. Previous studies on islet endothelial cells are extremely limited as it is difficult to isolatepure population of them. Thus, with this exciting discovery of highly purified islet endothelial cells derived from stem cells, the impact of these cells on insulin-producing cells can be investigated for a better understanding of interaction between neighboring cells, which may lead to a significant step in formulating new therapeutic angiogenic approaches for diabetes.
Columbia University, Ph.D in Biomedical Engineering (2007)
Columbia University, MS in Biomedical Engineering (2002)
Honors and Awards
2005 Late-Breaking Basic Science Abstract at American Heart Association Scientific Sessions
2003 Biomedical Engineering Society Graduate Student Research Award
2003 Biomedical Engineering Society Graduate Student Research Award
Assistant Professor, New Jersey Institute of Technology, Newark, NJ, July 2011-present
Associate Research Scientist, Yale University, New Haven, CT 2009-2011
Postdoctoral Associate, Yale University, New Haven, CT 2007-2009
Graduate Assistant, Columbia University, New York, NY, 2001-2007
Biomedical Engineering Society, member
American Heart Association, member
New York Academy of Science, member
Biophysical Journal, Molecular and Cellular Biomechanics, Nature Methods, Tissue Engineering, Circulation Research, Regenerative Medicine, Cardiovascular Pathology, Cells Tissues Organs, Journal of Tissue Engineering and Regenerative Medicine, Materials Express, Medical and Biological Engineering and Computing
Cardiovascular Tissue Engineering, Graduate level course, BME698
Mechanical Fundamentals of Biomedical Engineering, BME301
Cell and Biomaterial Engineering Laboratory, BME385
Maturation of human embryonic stem cell-derived cardiomyocytes in vitro using 3D engineered
tissue model systems, CT Stem Cell Research Grant (Highest Score)
Neha J, Lee EJ. “Islet endothelial cell-derived from mouse embryonic stem cells”, Cell Transplantation, 2005, In Press
Hitscherich P, Aphale A, Prabir P, Lee EJ. Biocompatibility of PCL-graphene electrospun scaffolds with mouse embryonic stem cell-derived cardiomyocytes, Tissue Engineering, In preparation
Hitscherich P, Wu S, Arinzeh T, Lee EJ. The effect of electrospun piezoelectric scaffold on mouse embryonic stem cell derived cardiomyocytes function, Tissue Engineering, In preparation
Maidhof R, Tandon N, Lee EJ, Luo J, Duan Y, Yeager K, Konofagou E,Vunjak-Novakovic G. “Biomimetic perfusion and electrical stimulation applied in concert improved the assembly of engineered cardiac tissue”, Journal of Tissue Engineering and Regenerative Medicine, 6(10): e12-23, 2012.
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. “Tissue-engineered lungs for in vivo implantation”, Science, 329(5991):538-41, 2010.
Lee EJ, Niklason LE. “A novel flow bioreactor for in vitro microvascularization”, Tissue Engineering Part C,16(5): 1191-200,2010.
Maidhof R, Marsano A, Lee EJ, Vunjak-Novakovic G. “Perfusion seeding of channeled channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineering”, Biotechnology Progress, 26(2):565-72, 2010.
Lee EJ, Vunjak-Novakovic G, Wang Y, Niklason LE. “A novel biocompatible endothelial cell delivery system for in vitro tissue engineering”, Cell Transplantation, 18(7):731-743, 2009.
Lee EJ, Holmes JW, Costa KD. “Remodeling of engineered tissues in response to altered boundary conditions”, Annals of Biomedical Engineering, 36(8): 1322-1334, 2008.
Lee EJ, Kim DE, Azeloglu EU, Costa KD. “Tissue engineering: Creation of a functional cardiac organoid”, Tissue Engineering, 14(2): 215-225, 2008 (accepted with no revision).
Kim DE, Lee EJ, Martens TP, Kara R, Chaudhry H, Itescu S, Costa KD. “Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms”, Conf Proc IEEE Eng Med Biol Soc 1:1545-7, 2006
Costa KD, Lee EJ, Holmes JW. “Creating alignment and anisotropy in engineered heart tissue: the role of boundary conditions in a model three-dimensional culture
systems”, Tissue Engineering, 9(4): 567-577, 2003 (with cover illustration).
Engineering cardiac tissue chambers as model systems for studying ventricular mechanics. Invention report M06-018 filed Sep. 1, 2005. Provisional patent application 60/721,399 filed Sep. 28.
Lee EJ. In vitro model systems for cardiovascular tissue engineering, Drexel University, Philadelphia, PA, March 15, 2010
Lee EJ. In vitro model systems for cardiovascular tissue engineering, New Jersey Institute of Technology, Newark, NJ, November 20, 2009
Lee EJ, Niklason LE. Preliminary study of microvascularization using a novel flow bioreactor, Yale Vascular Biology and Therapeutics Program Annual Retreat, New Haven, CT, November 15, 2008.
Lee EJ. Structural and functional charaterization of engineered cardiac tissue, Yale University July 6, 2006
Lee EJ. Structural and functional charaterization of engineered cardiac tissue, University of Pennsylvania, June 20, 2006
Published Conference Abstracts and Presentations
Hitscherich P, Aphale A, Prabir P, Lee EJ. Biocompatibility of PCL-graphene electrospun scaffolds with mouse embryonic stem cell-derived cardiomyocytes, TERMIS-AM, Washington D.C, VA, December 13-16, 2014
Lee EJ. Mouse embryonic stem cell-derived endothelial cells: towards vascularized engineered tissue, Rutgers New Jersey Medical School, NJ, February 17, 2014 (Invited Seminar)
Lee EJ. Stem cells and tissue engineering, City College of New York, NY, November 13, 2013 (Invited Seminar)
Jain NM, Lee EJ. I Jain NM, Lee EJ. Islet endothelial cells derived from mouse embryonic stem cells. The 8th NYSCF Conference, New York, NY, October 16-17, 2013
Jain NM, Haq-Siddiqi N, Lee EJ. A 3D microfluidic gel system for stem cell-derived endothelial cells, BMES Annual Fall meeting, Seattle, WA, September 25-29, 2013.
Marsh M, Arinzeh T, Lee EJ. The effect of electrospun piezoelectric scaffolds on endothelial cell growth, NJIT undergraduate Research Symposium, August 1, 2013.
Vejseli V, Lee EJ. Cardiac fibroblast-formed anisotropic decellularized engineered tissues, Northeast Bioengineering Conference, Syracuse, NY, April 5-7, 2013.
Jain NM, Lee EJ. Endothelial cells as a side-product from mouse embryonic stem cell differentiation into pancreatic islet cells, BMES CMBE Annual Meeting, Waimea, HI, January 2-5, 2013.
Vejseli V, Lee EJ. Cardiac fibroblast-formed anisotropic decellularized engineered cardiac tissues, BMES CMBE Annual Meeting, Waimea, HI, January 2-5, 2013.
Kim DE, Kao E Kang HR, Lee EJ, Costa KD. Global and regional response to cryo-injury in engineered cardiac tissues, BMES Annual Fall Meeting, Pittsburgh, PA, October 7-10, 2009.
Lee EJ, Kim DE, Azeloglu EU, Costa KD. Engineered cardiac organoid chambers for studying ventricular function, injury and repair, Tissue Engineereing International and Regenerative Medicine Society, San Diego, CA, December 7-10, 2008
Lee EJ, Niklason LE. Preliminary study of microvascularization using a novel in vitro flow bioreactor. The Eight Annual Retreat of Vascular Biology, and Therapeutics Program, New Haven, CT, November 15, 2008.
Kim DE, Lee EJ, Martens TP, Itescu S, Costa KD. Co-culture with mesenchymal precursor cells or cardiac fibroblasts augments contractile function of myocytes in 3-D engineered cardiac tissues, Society of Physical Regulation in Biology and Medicine, Miami, FL, January 9-11, 2008.
Kim DE, Lee EJ, Costa KD. A soft lithography device for long-term monitoring of contractile force in engineered cardiac tissues, BMES Annual Fall Meeting, Chicago, IL, October 11-14, 2006.
Kim DE, Lee EJ, Martens TP, Kara R, Chaudhry H, Itescu S, Costa KD. Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms, The 28th Annual International Conference IEEE Engineering in Medicine and Biology Society, New York, NY, August 31-September 3, 2006.
Lee EJ, Martens TP, Kim DE, Naseem TM, Itescu S, Costa KD. 3-D Engineered tissue model system for characterization of human mesenchymal stem cells with cardiomyocytes in co-culture, World Congress on Medical Physics and Biomedical Engineering, Seoul, Korea, August 27-September 1, 2006.
Lee EJ, Kim DE, Costa KD. Regional mechanics in an engineered tissue chamber model of myocardial infarction, Fifth World Congress of Biomechanics, Munich, Germany, July 29-August 4, 2006.
Lee EJ, Kim DE, Azeloglu EU, Costa KD. Engineered cardiac organoid: Developing a functional biologic micro-pump, The second International Conference on Cell Therapy for Cardiovascular Diseases, New York, NY, January 19-21, 2006.
Lee EJ, Kim DE, Azeloglu EU, Alexander C, Costa KD. Engineered cardiac tissue chambers demonstrate functional Frank-Starling mechanism and positive stroke work, American Heart Association Scientific Sessions, Dallas, TX, November 12-16, 2005 (Late-Breaking Basic Science Abstract).
Lee EJ, Kim DE, Azeloglu EU, Alexander C, Costa KD. Engineered cardiac tissue chambers as model systems for studying ventricular mechanics, BMES Annual Fall Meeting, Baltimore, MD, September 28-October 1, 2005.
Lee EJ, Kim DE, Alexander C, Costa KD. Pressure-volume and stress-strain relationships in engineered cardiac tissue chambers, ASME Annual Summer Conference, Vail, CO, June 22-26, 2005.
Costa KD and Lee EJ. Guiding cardiac tissue architecture by in vitro mechanical stimulation. Whitaker Biomedical Engineering Research Conference, San Diego, CA, August 13-15, 2004.
Kelly TN, Ng KW, Lee EJ, Lima E, Statman LY, Doty SB, Costa KD, Ateshian GA, Hung CT. Development of radial properties of engineered articular cartilage using unconfined compression loading. Trans 50th Annual Meeting of the ORS, San Francisco, CA, March 6-9, 2004.
Lee EJ, Holmes JW, Costa KD. Biaxial material properties of ribose treated collagen gels. BMES Annual Fall Meeting, Nashville, TN, October 1-4, 2003.
Lee EJ, Holmes JW, Costa KD. Remodeling of engineered tissues in response to altered boundary conditions. BMES Annual Fall Meeting, Nashville, TN, October 1-4, 2003 (BMES Graduate Student Award).
Lee EJ, Knezevic V, Holmes JW, Costa KD. Engineered tissues as model systems for studying myocardial biomechanics. Fourth World Congress of Biomechanics, Calgary, Canada, August 5-9, 2002.
Lee EJ, Costa KD. Boundary conditions determine fibroblast alignment in collagen gels. BMES Annual Fall Meeting, Durham, NC, October 4-7, Annals of Biomedical Engineering, 29:S-43-2001