Sagnik Basuray, PhD
Department of Chemical, Biological and Pharmaceutical Engineering
New Jersey Institute of Technology
ESSENCE - A novel shear-enhanced, flow-through nanoporous capacitive electrode based electrochemical platform
Conventional laboratory-based microarray technology is relatively slow, employs multistep protocols, and uses bulky and expensive fluorescent microscopy operated by trained staff restricting the use of such systems to laboratory settings. I will present here an electrochemical sensing method that uses shear-enhanced, flow-through nanoporous capacitive electrode (called ESSENCE). ESSENCE is highly sensitive and selective, negates biofouling, removes false positives and false negatives and has an electrochemical signal free from the parasitic double layer capacitor. ESSENCE has four advantages over current biosensors. First, the electrode nanoporosity allows for high shear forces of the order of a hydrogen bond to develop which tremendously increases the selectivity of the platform and allows sensor regeneration. Secondly, the electrochemical response is facilitated by the nanoporous geometry. The high signal to noise ratio of interdigitated microelectrodes tremendously increases sensitivity. Nanoconfinement effects boost the signal so high that ESSENCE can detect even surface functionalization of the electrode. Thirdly, the nanoporous geometry increases convective transport of the target to the bio-recognition element, reducing diffusion times and making the platform rapid. The convective transport enhancement from the high shear flow removes the parasitic double layer capacitance signal, allowing rapid measurements of the binding signal at the significantly reduced noise. Finally, because shear force can be varied by altering the flow rate, the selectivity and the sensitivity problem can be decoupled from each other using the shear force as a tuning mechanism. This decoupling allows us to mitigate the problems of biofouling, false positives, and false negatives. Using p53 as a target protein molecule, ESSENCE biosensor demonstrates a LOD of 5×10-16 M. with a limit of quantification of 3.63×10-16 M. Targeted nucleic acid detection tests are currently underway to show that the platform can detect at single base nucleotide levels. The EIS signature resulting from the docking of a multitude of biomolecules to CNTs shows the promise of the platform for a host of point-of-care diagnostic devices. The same platform can be extended to detection of chemicals also.
Sagnik is an assistant professor in the Department of Chemical, Biological and Pharmaceutical Engineering in the College of Engineering. He has been instrumental in developing lab on a chip technology for the last five years with collaborators from biology, biochemistry, biomedical and medicine. Sagnik is currently working collaboratively with many researchers in NJIT and at other National Labs (PNNL) and universities to stimulate novel, innovative and potentially high-risk research projects in nano/biotechnology areas (especially chemical and biological sensors) that exploit his groups’ expertise in nanotechnology platforms, nano/microfluidics, studying metal/dielectric/bio interfaces using SERS and plasmonics, electrodynamic and electrohydrodynamic simulations from bulk to nano. The current research thrusts including collaborative efforts are; (1) Shear enhanced discrimination based microfluidic CNT platform for (a) A flow-through nanoporous capacitive electrode based sensor for sensing of cancer biomarkers (Dr. Stephen Suh, Hackensack Medical Center), Organic Halides and Radioactive Traces in groundwater (Dr. Sayandev Chatterjee, PNNL), (b) Simultaneous separation, purification and investigation of dynamics of monoclonal antibodies (mAbs) (Dr. Kamalesh Sirkar and Dr. Somenath Mitra, NJIT) (2) Control of Root Growth and Ecosystems for Healthy Living Environments (Prof. Mathew Schwartz (NJIT), NAAVA). (3) Antibacterial study of Chalcogenide Perovskite Materials and Semiconductors like ReS2 (Dr. Nikhil Koratkar, RPI). Sagnik has been successful in the development and commercialization of technology through the license of multiple patents (Licensee: http://cubedlabs.com). In his short academic career, he has published papers in top journals like Lab-on-a-Chip, ACS Nano, Physical Review, Langmuir with an h-index of 9 and over 3 patents. Sagnik recently received the National Science Foundation CAREER award in Biosensing for 2018.